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2018 Vol.43, Issue 4 Preview Page

Original Article

Evaluation of SERS Nanoparticles to Detect Bacillus cereus and Bacillus thuringiensis
Jeehwa Hong12
Jianwei Qin1
Jo Ann S Van Kessel1
Mirae Oh1
Sagar Dhakal1
Lee Hoonsoo3
Chansong Hwang1
Diane E Chan1
Dongho Kim2
Hyunjeong Cho2*
Moon S Kim1*
1Environmental Microbial and Food Safety Laboratory, Agricultural Research Service, US Department of Agriculture, MD 20705, USA
2Experiment Research Institute, National Agricultural Products Quality Management Service, Gimcheon 39660, Korea
3Dept. of Biosystems Engineering, Chungbuk National University, Cheongju 28644, Korea
* Corresponding Author
* Corresponding Author
December 2018. pp. 394-400
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Abstract

Purpose: This research evaluated five types of nanoparticles to develop a surface-enhanced Raman spectroscopy (SERS) method for the rapid detection of two Bacillus species (Bacillus cereus and Bacillus thuringiensis) that are commonly found on fresh produce, which can cause food poisoning. Methods: Bacterial concentrations were adjusted to a constant turbidity, and a total of 30 μL of each Bacillus cell suspension was prepared for each nanoparticle. A point-scan Raman system with laser light source of wavelength 785 nm was used to obtain SERS data. Results: There was no qualitative difference in the SERS data of B. cereus and B. thuringiensis for any of the five nanoparticles. Three gold nanoparticles, stabilized in either citrate buffer or ethanol, showed subtle differences in Raman intensities of two Bacillus species at 877.7 cm-1. Conclusions: Among the three types of nanoparticles, the gold nanoparticles stabilized in citrate buffer showed the lowest standard deviation, followed by gold nanoparticles stabilized in ethanol. This result supports the potential application of gold nanoparticles for SERS-based detection of B. cereus and B. thuringiensis.

Keywords
Bacillus cereus
Bacillus thuringiensis
Nanoparticle
Point-scan Raman
SERS
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Information
  • Publisher :The Korean Society for Agricultural Machinery
  • Publisher(Ko) :한국농업기계학회
  • Journal Title :Journal of Biosystems Engineering
  • Journal Title(Ko) :바이오시스템공학
  • Volume : 43
  • No :4
  • Pages :394-400
  • Received Date :2018. 09. 28
  • Revised Date :2018. 11. 09
  • Accepted Date : 2018. 11. 11
  • DOI :https://doi.org/10.5307/JBE.2018.43.4.394
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